Expression of survivin, caspase-3 in oral precancerous lesions and oral squamous-cell carcinoma
10.3760/cma.j.issn.1002-0098.2010.02.006
- VernacularTitle:生存素、半胱氨酸天冬氨酸蛋白酶3在口腔癌前病变及口腔癌中的表达
- Author:
Yan-Ping DING
1
;
Shu-Xia LI
;
Hong-Ru WU
;
Xin-Yan ZHANG
;
Xiao-Fei TANG
;
Zheng SUN
Author Information
1. 北京老年医院
- Keywords:
Caspase 3;
Mouth neoplasms;
Survivin;
Apoptosis
- From:
Chinese Journal of Stomatology
2010;45(2):85-88
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the roles of surviving and caspase-3 in the development of oral cancer. Methods Archival tissue sections of 17 oral squamous cell carcinoma(OSCC), 28 oral leukoplakia with dysplasia, 10 normal oral mucosa were obtained from Capital Medical University School of Stomatology for immunohistochemical staining of markers of survivin and caspase-3. The cell apoptosis was detected with terminal dooxynucleotidyl transferase-mediated nucleotide shift enzyme (TdT) mediated d-UTP end labeling (TUNEL). Positively stained cells were counted and analyzed statistically to determine potential relationship between survivin, caspase-3 and cell apoptosis. Results The expression of survivin was faint or negative in normal epithelial cells. The average positive rate of survivin was (1.05± 1.21)% in control group and (21.89 ±10.45)% in OSCC. Caspase-3 was expressed in all the normal mucosa, but it obviously down-regulated in dysplasia and OSCC. The apoptosis index (AI) decreased from (0. 89 ± 0. 46) % in normal mucosa to (0. 21±0. 12)% in OSCC. Conclusions Both survivin and caspase-3 are associated with carcinogenesis of the oral mucosa. Survivin may restrain cell apoptosis by inhibiting caspase-3.
