Effects of Podoplanin on cell proliferation and cell cycle in oral leukoplakia cells.
- Author:
Qiang SUN
1
;
Ming YAN
;
Xiao-jian ZHOU
;
Xin-ming LI
;
Li MAO
;
Wan-tao CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Western; Cell Cycle; Cell Line, Tumor; Cell Proliferation; Flow Cytometry; Humans; Leukoplakia, Oral; metabolism; pathology; Membrane Glycoproteins; genetics; metabolism; physiology; RNA, Messenger; metabolism; Real-Time Polymerase Chain Reaction; Transfection
- From: Chinese Journal of Stomatology 2010;45(1):6-10
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of Podoplanin on cell proliferation and cell cycle in oral leukoplakia cells.
METHODSOral leukoplakia cell line (Leuk-1) transfected with pCMV-Podoplanin (A4-1) or pCMV (B4-1) was used in this study. The levels of mRNA and protein of Podoplanin were detected by real-time PCR and Western boltting in A4-1, B4-1 and Leuk-1 cells. The localization of Podoplanin was detected by confocal microscope. Cell growth and proliferation was examined by methyl thiazolyl tetrazolium (MTT) assay and cell cycle was detected by flow cytometry (FCM).
RESULTSThe value of Podoplanin mRNA level in A4-1 cells was 0.022, which was significantly higher than the values in B4-1 (0.001) and Leuk-1 cells (0.002), P < 0.05. The gray scale of Podoplanin protein in A4-1 cells was significantly higher than in the control groups (P < 0.05). The expression of Podoplanin was observed in cell plasm and membrane of A4-1, B4-1 and Leuk-1 cells. But the expression level of Podoplanin in A4-1 cells was higher than in control groups. A4-1 cells grew faster than Leuk-1 cells. The proliferation rate after 3 days of culture in A4-1 cells was 40.4% higher than that in B4-1 cells (P < 0.05). The G₂-M phase (24.89 +/- 4.55)% and PI (0.57 +/- 0.06) of A4-1 cells were significantly higher than that in B4-1 cells [(4.13 +/- 5.24)%, (0.41 +/- 0.04)] and Leuk-1 cells [(4.69 +/- 7.42)%, (0.40 +/- 0.02)], P < 0.05.
CONCLUSIONSOver expression of Podoplanin accelerated the growth and proliferation of oral leukoplakia cells. Podoplanin may be one of key genes in the malignant transformation of oral leukoplakia.
