Effects of Podoplanin on cell proliferation and cell cycle in oral leukoplakia cells
10.3760/cma.j.issn.1002-0098.2010.01.003
- VernacularTitle:转染Podoplanin对口腔白斑细胞增殖和细胞周期的影响
- Author:
Qiang SUN
1
;
Ming YAN
;
Xiao-Jian ZHOU
;
Xin-Ming LI
;
Li MAO
;
Wan-Tao CHEN
Author Information
1. 郑州大学附属第一医院
- Keywords:
Leukoplakia,oral;
Cell proliferation;
Cell cycle;
Podoplanin
- From:
Chinese Journal of Stomatology
2010;45(1):6-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Pedoplanin on cell proliferation and cell cycle in oral leukoplakia cells.Methotis Oral leukoplakia cell line(Leuk-I)transfected with pCMV-Podoplanin (A4-1)or pCMV(B4-1)was used in this study.The levels of mRNA and protein of Podoplanin were detected by real-time PCR and Western boltting in A4-1,B4-1 and Leuk-1 cells.The localization of Podoplanin was detected by confocal microscope.Cell growth and proliferation was examined by methyl thiazolyl tetrazolium(MTT)assay and cell cycle was detected by flow cytometry(FCM).Results The value of Podoplanin mRNA level in A4-1 cells was 0.022.which was significantly higher than the values in B4-1 (0.001)and Leuk-1 cells(0.002),P<0.05.The gray scale of Podoplanin protein in A4-1 cells was significantly higher than in the control groups(P<0.05).The expression of Podoplanin was observed in cell plasm and membrane of A4-1,B4-1 and Leuk-1 cells.But the expression level of Podoplanin in A4-1 cells was higher than in control groups.A4-1 cells grew faster than Leuk-1 cells.The proliferation rate after 3 days of culture in A4-1 cells was 40.4% higher than that in B4-1 cells(P<0.05).The G_2-M phase(24.89±4.55)% and PI(0.57±0.06)of A4-1 cells were significantly higher than that in B4-1 cells[(4.13±5.24)%,(0.41±0.04)]and Leuk-1 cells[(4.69±7.42)%,(0.40±0.02)],P<0.05.Conclusions Over expression of Podoplanin accelerated the growth and proliferation of oral leukoplakia cells.Podoplanin may be one of key genes in the malignant transformation of oral leukoplakia.
