Expression of caspase-8,receptor interacting protein and nuclear factor-kBp65 in oral lichen planus
10.3760/cma.j.issn.1002-0098.2010.01.004
- VernacularTitle:半胱氨酸天冬氨酸蛋白酶8、受体作用蛋白及核因子-κBp65在口腔扁平苔藓中的表达及意义
- Author:
Yong-Jian SHI
1
;
Li-Jia SHEN
;
Cao YIN
Author Information
1. 广州医学院口腔医院
- Keywords:
Lichen planus,oral;
Caspase-8;
NF-kappaB;
Receptor interacting protein
- From:
Chinese Journal of Stomatology
2010;45(1):11-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of caspase-8,receptor interacting protein (RIP) and nuclear factor(NF)-kBp65 in oral lichen planus(OLP)and their relationship with cell apoptosis.Methods Immunohistochemical technique with SP method was used to detect the expressions of caspase-8,RIP and NF-kBp65 in 30 OLP cases and 15 normal oral mucosa specimens.Terminal deoxynucleotidyl transferase-mediated nucleotide shift enzyme(TdT)mediated d-UTP end labeling(TUNEL)was used for detecting the cell apoptotic index(AI)in 15 OLP cases and 5 nomal oral mucosa specimens.Results Compared with the control group,the AI of epithelial cells(6.76±2.32)increased and the AI of lymphocytes(1.75±0.74)decreased in OLP(P<0.01).The positive rate of caspase-8,RIP and NF-kBp65 OLP than in normal control(P<0.05).The positive rate of caspase-8,RIP and NF-kBp65 of lymphocytes control(P<0.01).A positive correlation was also observed between NF-kBp65 expression of lymphocytes and AI of epithelial cells.Conclusions Accelerated apoptosis of the keratinocytes and inhibition of lymphocyte apoptosis may coexist and contribute to the formation and progression of OLP.The over expression of caspase-8,RIP and NF-kBp65 in OLP may play a role in the pathogenesis of OLP.
