Effects of lactoferrin on vascular endothelial growth factor and basic fibroblast growth factor expression in human Tca8113 cell line.
- Author:
Lei WANG
1
;
Xiu-feng WEI
;
Hong-chen SUN
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; administration & dosage; pharmacology; Carcinoma, Squamous Cell; genetics; metabolism; pathology; Cell Line, Tumor; Dose-Response Relationship, Drug; Down-Regulation; Fibroblast Growth Factor 2; genetics; metabolism; Humans; Lactoferrin; administration & dosage; pharmacology; RNA, Messenger; metabolism; Tongue Neoplasms; genetics; metabolism; pathology; Vascular Endothelial Growth Factor A; genetics; metabolism
- From: Chinese Journal of Stomatology 2010;45(1):28-30
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of lactoferrin on vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) expression.
METHODSLactoferrin at concentration of 0.006, 0.013, 0.025, 0.050 g/L and blank control groups were included in this study. The gene and protein expression of VEGF, bFGF were examined by RT-PCR and Western blotting.
RESULTSThe RT-PCR and Western blotting assay showed that VEGF mRNA(0.31 +/- 0.08) and protein (0.68 +/- 0.11) in lactoferrin (0.050 g/L) group were significantly lower than in the control group (P < 0.05), and the bFGFmRNA (0.27 +/- 0.10) and protein (0.68 +/- 0.07) in lactoferrin (0.050 g/L) group were also significantly lower than in the control group (P < 0.05).
CONCLUSIONSLactoferrin could inhibit the expression of VEGF, bFGFmRNA and protein in Tca8113 cells. This effect might be one of the mechanisms for anticancer function of lactoferrin.
