Effect of microRNA on proliferation of human tongue carcinoma Tca8113 cells
10.3760/cma.j.issn.1002-0098.2010.03.006
- VernacularTitle:微小RNA对人舌鳞状细胞癌Tca8113细胞增殖的影响
- Author:
Bing-Xiu XIAO
1
;
Jun-Ming GUO
;
Jiu-Chang ZHONG
Author Information
1. 宁波大学医学院
- Keywords:
Carcinoma,squamous cell;
MicroRNA;
Cell growth processes
- From:
Chinese Journal of Stomatology
2010;45(3):152-154
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of microRNA(miRNA)on proliferation of cultured human squamous cell carcinoma of tongue Tca8113 cells.Methods The mimics or inhibitors of miRNA-31 or miRNA-139 were transfected into Tca8113 cells using liposome.Tca8113 cell proliferation was detected by 3-(4,5-dimethyhhiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.Results The absorbance (A) values of control group at 24 h,48 h and 72 h were 0.125±0.002,0.169±0.002 and 0.216±0.004.respectively.The mimics of miRNA-31 increased Tca8113 cell proliferation.with A values inereasing to 0.136±0.001(P<0.001).0.186±0.004(P<0.001)and 0.249±0.012(P<0.01),respectively.The inhibitors of miRNA-139 also inereased A values to 0.148±0.002(P<0.001).0.214±0.002(P<0.001)and 0.250±0.009(P<0.01),respectively.Contrast with these results,the inhibitors of miRNA-31 decreased Tca8113 cell proliferation,with A values decreasing to 0.145 4-0.001 and 0.155±0.011 (both of P<0.001) at 48 h and 72 h,respectively.The mimics of miRNA-139 also decreased A to 0.135±0.001 and 0.170±0.009 (both of P<0.001).Conclusions miRNA-31 and miRNA-139 play an important role in the carcinogenesis of human tongue carcinomas.It may become a new method for the treatment of tongue carcinomas by adjustment the activities of miRNA.
