Expression of Fas and Fas ligand in infiltrating lymphocytes in patients with oral lichen planus
10.3760/cma.j.issn.1002-0098.2010.04.007
- VernacularTitle:口腔扁平苔藓固有层淋巴细胞中Fas及Fas配体的表达变化
- Author:
Lei LEI
1
;
Wei-Xia TAN
;
Xu-Long ZHOU
;
Pei-E ZHENG
Author Information
1. 暨南大学医学院
- Keywords:
Lichen planus,oral;
Fas ligang protein;
Activation-induced cell death
- From:
Chinese Journal of Stomatology
2010;45(4):219-222
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the expression of Fas and Fas ligand(FasL)in T lymphocytes of oral lichen planus(OLP)and the effects of Fas,FasL and activation-induced cell death(AICD)on OLP.Methods The oral mucosa samples from patients with OLP and nomal oral mucosa were assessed by in situ terminal deoxynucleotidyl transferase(TdT)-mediated deoxvuridine-5'-triphosphate(dUTP)-biotin nick endlabelling(TUNEL)assay for nucleosomal DNA fragmentation of apoptotic cells in infiltrating lymphocytes.Immunohistochemcial technique was used for detection of expression level of Fas and FasL.Immunohistochemcial double labeling was performed to examine the expression of Fas and FasL in CD8~+ T cells and CD4~+ T cells.Results The rate of apoptosis in infiltrating lymphocytes of OLP was(1.9±1.8)%,which was lower than that of normal oral mucosa(P=0.013).The expression of Fas and FasL were increased in lymphocytes of OLP(P=0.005 and 0.000 respectively).The positive rate of double labeled cells of CD8~+ and Fas~+ was not increased in OLP(P=0.313),and of CD8~+ and FasL~+ in OLP was higher than that of normal oral mucosa(P=0.002).The expression of double labeled cells of CD4~+ and Fas~+ in OLP was higher than that of control group(P=0.031).The expression of CD4~+ and Fas~+ T cells in reticular OLP were increased(P=0.019),and of CD4+ and FasL+ did not show remarkable change(P=0.097).Conclusions There was a low frequency of lymphocytic apoptosis.T cells,especial CD8~+ T cells in OLP and CD4~+ T cells in atrophic-erosive OLP appeared to escape from AICD,which may account for the persistense of inflammation.Some CD4~+ T cells in reticular OLP may go through AICD.
