Effects of Porphyromonas gingivalis lipopolysaccharide on apoptotic genes in foam cells.
- Author:
Hou-xuan LI
1
;
Fu-hua YAN
;
Lang LEI
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Caspase 3; metabolism; Cell Line, Tumor; Cell Proliferation; drug effects; Foam Cells; cytology; drug effects; metabolism; Gene Expression; Humans; Lipopolysaccharides; isolation & purification; pharmacology; Lipoproteins, LDL; pharmacology; Macrophages; physiology; Minor Histocompatibility Antigens; Porphyromonas gingivalis; chemistry; Proto-Oncogene Proteins c-bcl-2; metabolism; Proto-Oncogene Proteins c-myc; metabolism; Tumor Suppressor Protein p53; metabolism
- From: Chinese Journal of Stomatology 2010;45(5):274-278
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) on apoptotic genes in foam cells.
METHODSMacrophages from THP-1 monocytes and foam cells from macrophages by oxLDL inducement were treated with oxidized low density lipoprotein (oxLDL) or oxLDL+ Pg-LPS. Cell apoptosis was detected by acridine orange-ethidium bromide (AO-EB) staining. Eleven atherosclerotic related apoptotic genes were examined with polymerase chain reaction (PCR) array, and apoptotic gene p53, c-Myc and caspase-3 were evaluated with real-time PCR.
RESULTSPg-LPS enhanced cell apoptosis rate during and after foam cells formation [(5.47+/-0.93)% vs. (7.50+/-0.54)%]. PCR array demonstrated that it increased B-cell CLL-lymphoma 2 (BCL2) related protein A1 (BCL2A1) transcription during foam cells formation (>2 fold), and promoted BCL2 and BCL2A1 transcription after foam cells formation (>2 fold). It promoted p53 and caspase-3 transcription level (4.50x10(-3)+/-4.02x10(-4) vs. 5.30x10(-2)+/-4.58x10(-3)), whereas inhibited c-Myc transcription level (1.53x10(-2)+/-5.77x10(-4)) during foam cells formation. It promoted caspase-3 transcription (6.00x10(-2)+/-6.08x10(-3)), and inhibited p53 transcription (4.23x10(-3)+/-5.85x10(-4)) after foam cells formation.
CONCLUSIONSPg-LPS affected apoptotic gene transcription during and after foam cells formation and enhanced cell apoptosis.
