Effects of Porphyromonas gingivalis lipopolysaccharide on apoptotic genes in foam cells
10.3760/cma.j.issn.1002-0098.2010.05.005
- VernacularTitle:牙龈卟啉单胞菌脂多糖对泡沫细胞凋亡基因的影响
- Author:
Hou-Xuan LI
1
;
Fu-Hua YAN
;
Lang LEI
Author Information
1. 福建医科大学附属口腔医院
- Keywords:
Porphyromonas gingivalis;
Lipopolysaccharides;
Foam cells;
Apoptosis
- From:
Chinese Journal of Stomatology
2010;45(5):274-278
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) on apoptotic genes in foam cells.Methods Macrophages from THP-1 monocytes and foam cells from macrophages by oxLDL inducement were treated with oxidized low density lipoprotein (oxLDL) or oxLDL + Pg-LPS.Cell apoptosis was detected by acridine orange-ethidium bromide (AO-EB) staining.Eleven atherosclerotic related apoptotic genes were examined with polymerase chain reaction (PCR) array, and apoptotic gene p53, c-Myc and caspase-3 were evaluated with real-time PCR.Results Pg-LPS enhanced cell apoptosis rate during and after foam cells formation[(5.47±0.93) % vs.(7.50±0.54) %].PCR array demonstrated that it increased B-cell CLL-lymphoma 2 (BCL2) related protein Al (BCL2A1) transcription during foam cells formation (>2 fold), and promoted BCL2 and BCL2A1 transcription after foam cells formation (> 2 fold).It promoted p53 and caspase-3 transcription level (4.50 × 10-3±4.02 × 10-4 vs.5.30 × 10-2±4.58 × 10-3), whereas inhibited c-Myc transcription level (1.53 × 10-2±5.77 × 10-4) during foam cells formation.It promoted caspase-3 transcription (6.00 × 10-2±6.08 × 1-3), and inhibited p53 transcription (4.23 × 10-3±5.85 × 10-4) after foam cells formation.Conclusions Pg-LPS affected apoptotic gene transcription during and after foam cells formation and enhanced cell apoptosis.
