Screening of virulence gene in golden hamster cheek poch mucosa carcinomatous change induced by 9,10-dimethylen-1,2-benzanthracene
10.3760/cma.j.issn.1002-0098.2010.05.013
- VernacularTitle:苯并蒽诱导金黄地鼠颊黏膜癌变的重要致病基因筛选
- Author:
Guo-Dong ZHANG
1
;
Kai YANG
;
Jie MEI
Author Information
1. 重庆医科大学附属第一医院
- Keywords:
Mouth mucosa;
Carcinoma,squamous cell;
Genes
- From:
Chinese Journal of Stomatology
2010;45(5):303-306
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine and analyze the global gene expression at the different stages of golden hamster cheek poch mucosa carcinomatous change induced by 9,10-dimethylen-1 ,2 benzanthracene (DMBA).Methods The model of golden hamster cheek poch squamous cell carcinoma was induced by DMBA.The RNA of normal mucosa, precancerous lesions and squamous cell carcinoma of fresh tissue of golden hamsters was extracted and purified and the cRNA labeled by fluorescent Cy3 synthesized, which respectively hybridized with the agilent rat cDNA microarray containing 41 000 genes-expressed sequence tags, scanning with Agilent G2565AA fluorescence scanner.The Ratio ≥2 and Ratio ≤0.5 were used to determine the threshold value of the differences gene expression and analyze the relevant biological information of the three different stages of cancer.The Venn diagram by Gene Spring 10.0 analysis software was then used to select the genes of continuing expression in the three different stages of cancer.Meanwhile, RT-PCR was used to confirm the correctness of the results of the gene chip.Results A total of 5255 differentially expressed genes were detected during the process from normal mucosa to the squamous cell carcinoma,of which 2896 was up-regulated and 2359 down-regulated.There were 22 genes that showed continues abnormal expression through the three different stages of carcinomatous change, including 3 up-regulated , 19 down-regulated.The RT-PCR results of Eaf-2 and Ecg-2 were consistent with the gene chip.Conclusions The development of oral mucosa]squamous cell carcinoma involved a number of abnormal genes.The genes showing continues abnormal expression at different stages of carcinomatous change may be the important pathogenetic ones.
