Effect of electroacupuncture at "Neiguan" (PC 6) on expression of c-fos gene in myocardium of the rat with acute myocardial ischemia.
- Author:
Fang XIE
1
;
Xun-Chang LIANG
;
Hong-Jin WU
;
Li-Qiang RU
Author Information
- Publication Type:Journal Article
- MeSH: Acupuncture Points; Animals; Electroacupuncture; Genes, fos; Humans; Myocardial Ischemia; genetics; Myocardium; metabolism; Rats
- From: Chinese Acupuncture & Moxibustion 2005;25(5):355-358
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the mechanism of electroacupuncture at "Neiguan" (PC 6) improving acute myocardial ischemia.
METHODSThe rats were randomly divided into a sham operation group, a myocardial ischemia model group and a myocardial ischemia model plus electroacupuncture group. The acute myocardial ischemia model was developed byligation of the descending anterior branch of the coronary artery, and electroacupuncture was given at bilateral "Neiguan" (PC 6). Serum myocardial enzymes was determined by biochemical method and the expression of c-fos mRNA in myocardium was detected by using reverse transcription polymerase chain reaction (RT-PCR).
RESULTSThe activities of serum myocardial enzymes and the expression of c-fos mRNA in ischemic myocardium were significantly increased as compared with those in the sham operation group (P < 0.05), and after electroacupuncture they were significantly decreased (P < 0.05).
CONCLUSIONThe mechanism of electroacupuncture at "Neiguan" (PC 6) improving acute myochadial ischemia is possibly related with down-regulation of expression of c-fos mRNA in myocardium.
