Relationship between artesunate influence on the process of TGF-beta1 induced alveolar epithelial cells transform into mesenchymal cells and on idiopathic pulmonary fibrosis.
- Author:
Chang-Ming WANG
;
Juan CHEN
;
Ming JIANG
;
Xiu-Ping XUAN
;
Hong-Xiu LI
- Publication Type:Journal Article
- MeSH:
Actins;
genetics;
metabolism;
Animals;
Artemisia;
chemistry;
Artemisinins;
isolation & purification;
pharmacology;
Cell Line;
Cell Proliferation;
drug effects;
Epithelial Cells;
cytology;
metabolism;
Epithelial-Mesenchymal Transition;
drug effects;
Idiopathic Pulmonary Fibrosis;
pathology;
Plants, Medicinal;
chemistry;
Pulmonary Alveoli;
cytology;
RNA, Messenger;
metabolism;
Rats;
Smad3 Protein;
genetics;
metabolism;
Smad7 Protein;
genetics;
metabolism;
Transforming Growth Factor beta1;
pharmacology;
Vimentin;
genetics;
metabolism
- From:
Acta Pharmaceutica Sinica
2014;49(1):142-147
- CountryChina
- Language:Chinese
-
Abstract:
This study is to investigate the effect of artesunate on transforming growth factor-beta1 (TGF-beta1) induced epithelial-mesenchymal transition (EMT) and its possible mechanism. After the in vitro cultured RLE-6TN cells were treated with TGF-beta1 then artesunate intervened on it, after 24 h, expression of the markers of mesenchymal cell was assayed using Western blotting and real-time PCR analysis. Western blotting was also used to detect the effect of TGF-beta1 on the Smad3 and Smad7 expressions of RLE-6TN cells. Morphological alterations were examined by phase-contrast microscope, and ultrastructure changes by electron microscope. Incubation of RLE-6TN cells with TGF-beta1 resulted in the up-regulation of the expression of the mesenchymal cell markers, after artesunate intervened on it, resulted in the down-regulation of the expression. Meanwhile, incubation with artesunate intervened on RLE-6TN cells could lead to the apparent down-regulation of the expression of Smad3 and up-regulation of Samd7 and the transition of RLE-6TN cells to mesenchymal-like by TGF-beta1 induction, after artesunate intervened on it, RLE-6TN cells to epithelial-like. TGF-beta1 induced epithelial-mesenchymal transition process; artesunate can inhibit TGF-beta1-induced epithelial-mesenchymal transition process, the possible mechanism is up-regulation of the expression of Smad7 and down-regulation of the expression of Smad3, meanwhile inhibits phosphorylation of Smad3.
