Anti-atherosclerosis role of N-oleoylethanolamine in CB2.
- Author:
Ya-Ting GAI
;
Qiang SHU
;
Cai-Xia CHEN
;
You-Lin LAI
;
Wen-Jun LI
;
Lu PENG
;
Li-Min LIN
;
Xin JIN
- Publication Type:Journal Article
- MeSH:
Anti-Inflammatory Agents;
pharmacology;
Atherosclerosis;
pathology;
Cell Adhesion;
drug effects;
Cells, Cultured;
Endocannabinoids;
pharmacology;
Endothelial Cells;
cytology;
metabolism;
Ethanolamines;
pharmacology;
Humans;
Indoles;
pharmacology;
Monocytes;
drug effects;
Oleic Acids;
pharmacology;
PPAR alpha;
antagonists & inhibitors;
RNA, Messenger;
metabolism;
Receptor, Cannabinoid, CB2;
antagonists & inhibitors;
genetics;
metabolism;
Tumor Necrosis Factor-alpha;
pharmacology;
Vascular Cell Adhesion Molecule-1;
metabolism
- From:
Acta Pharmaceutica Sinica
2014;49(3):316-321
- CountryChina
- Language:Chinese
-
Abstract:
To observe a PPAR-alpha agonist effect of N-oleoylethanolamine (OEA) on CB2 (cannabinoid receptor 2), an anti-inflammatory receptor in vascular endothelial cell, healthy HUVECs and TNF-alpha induced HUVECs were used to establish a human vascular endothelial cell inflammatory model. Different doses of OEA (10, 50 and 100 micromol x L(-1)) had been given to HUVECs, cultured at 37 degrees C for 7 h and then collected the total protein and total mRNA. CB2 protein expression was detected by Western blotting and CB2 mRNA expression was assayed by real-time PCR. As the results shown, OEA (10 and 50 micromol x L(-1)) could induce the CB2 protein and mRNA expression, but not 100 micromol x L(-1). To detect if anti-inflammation effect of OEA is partly through CB2, CB2 inhibitor AM630 was used to inhibit HUVEC CB2 expression, then the VCAM-1 expression induced by TNF-alpha was detected, or THP-1 adhere to TNF-alpha induced HUVECs was examined. OEA (50 micromol x L(-1)) could inhibit TNF-alpha induced VCAM-1 expression and THP-1 adhere to HUVECs, these effects could be partly inhibited by a CB2 inhibitor AM630. The anti-inflammation effect of OEA is induced by PPAR-alpha and CB2, suggesting that CB2 signaling could be a target for anti-atherosclerosis, OEA have wide effect in anti-inflammation, it may have better therapeutic potential in anti-inflammation in HUVECs, thus achieving anti-atherosclerosis effect.
