Inhibitory effect of arctigenin on lymphocyte activation stimulated with PMA/ionomycin.
- Author:
Cheng-Hong SUN
;
Xin-Qiang LAI
;
Li ZHANG
;
Jing-Chun YAO
;
Yong-Xia GUAN
;
Li-Hong PAN
;
Ying YAN
- Publication Type:Journal Article
- MeSH:
Animals;
Anti-Inflammatory Agents;
isolation & purification;
pharmacology;
Antigens, CD;
metabolism;
Antigens, Differentiation, T-Lymphocyte;
metabolism;
Arctium;
chemistry;
Cell Cycle Checkpoints;
drug effects;
Cell Proliferation;
drug effects;
Cytokines;
metabolism;
Female;
Furans;
isolation & purification;
pharmacology;
Interferon-gamma;
metabolism;
Interleukin-10;
metabolism;
Interleukin-2;
metabolism;
Interleukin-2 Receptor alpha Subunit;
metabolism;
Interleukin-4;
metabolism;
Interleukin-6;
metabolism;
Ionomycin;
pharmacology;
Lectins, C-Type;
metabolism;
Lignans;
isolation & purification;
pharmacology;
Lymphocyte Activation;
drug effects;
Mice;
Mice, Inbred BALB C;
Plants, Medicinal;
chemistry;
T-Lymphocytes;
cytology;
drug effects;
immunology;
Tetradecanoylphorbol Acetate;
pharmacology;
Tumor Necrosis Factor-alpha;
metabolism
- From:
Acta Pharmaceutica Sinica
2014;49(4):482-489
- CountryChina
- Language:English
-
Abstract:
This study investigated the effect of arctigenin (Arc) on the cell activation, cytokines expression, proliferation, and cell-cycle distribution of mouse T lymphocytes. Mouse lymphocytes were prepared from lymph node and treated with Phorbol-12-myristate-13-acetate (PMA)/Ionimycin (Ion) and/or Arc. CD69, CD25, cytokines, proliferation and cell cycle were assayed by flow cytometry. The results showed that, at concentrations of less than 1.00 micromol x L(-1), Arc expressed non-obvious cell damage to cultured lymphocytes, however, it could significantly down-regulate the expression of CD69 and CD25, as well as TNF-alpha, IFN-gamma, IL-2, IL-4, IL-6 and IL-10 on PMA/Ion stimulated lymphocytes. At the same time, Arc could also inhibit the proliferation of PMA/Ion-activated lymphocytes and exhibited lymphocyte G 0/G1 phase cycle arrest. These results suggest that Arc possesses significant anti-inflammatory effects that may be mediated through the regulation of cell activation, cytokines expression and cell proliferation.
