Effect of beclin1 on vincristine-induced dopaminergic neurons injury in zebrafish.
- Author:
Zhan-Ying HU
;
Jing-Pu ZHANG
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis Regulatory Proteins;
metabolism;
Autophagy;
Dopaminergic Neurons;
drug effects;
pathology;
Dose-Response Relationship, Drug;
Down-Regulation;
Gene Expression Regulation;
drug effects;
Larva;
drug effects;
Tyrosine 3-Monooxygenase;
metabolism;
Vincristine;
adverse effects;
Zebrafish;
Zebrafish Proteins;
metabolism
- From:
Acta Pharmaceutica Sinica
2014;49(6):843-848
- CountryChina
- Language:Chinese
-
Abstract:
To investigate vincristine-induced dopaminergic neurons toxicity and mechanism, and explore the molecular target to reduce the toxicity, zebrafish was chosen as a model animal, based on RT-PCR, Western blotting, whole mount in situ immunofluorescence and other technical means. The results showed that the transcription levels of tyrosine hydroxylase gene and dopamine transporter protein gene were inhibited. Furthermore, the number of dopaminergic neurons was decreased by vincristine. Autophagy was suppressed and beclin1 gene expression was inhibited in a dose-dependent manner by vincristine in larval zebrafish. Up-regulated beclin1 partly reduced vincristine-induced neurotoxicity, and down-regulated beclin1 increased toxicity. Beclin1 plays an important role in vincristine-induced dopaminergic neurons toxicity.
