A cyclotide against influenza A H1N1 virus from Viola yedoensis.
- Author:
Min-Zhi LIU
;
Yan YANG
;
Shu-Xiang ZHANG
;
Liang TANG
;
Hui-Min WANG
;
Cheng-Juan CHEN
;
Zhu-Fang SHEN
;
Ke-Di CHENG
;
Jian-Qiang KONG
;
Wei WANG
- Publication Type:Journal Article
- MeSH:
Antiviral Agents;
isolation & purification;
pharmacology;
Cyclotides;
pharmacology;
Influenza A Virus, H1N1 Subtype;
drug effects;
Tandem Mass Spectrometry;
Viola;
chemistry
- From:
Acta Pharmaceutica Sinica
2014;49(6):905-912
- CountryChina
- Language:Chinese
-
Abstract:
Three cyclotides were isolated from the whole plant of Viola yedoensis in this study. The two, vary peptide E and cycloviolacin Y5, were previously reported, and a novel cycloviolacin VY1 was characterized according to the interpretation of MS/MS fragmentation of peptides which were produced from the reduced and alkylated parent peptide with the digestion of Endo Lys-C, trypsin and chymotrypsin, separately. The stability of remarkable resistance to proteolytic degradation by trypsin and chymotrypsin, and that of thermal denaturation was confirmed again. Besides, the IC50 value of cycloviolacin VY1 against influenza A H1N1 virus was (2.27 +/- 0.20) microg x mL(-1). It is the first cyclotide reported with anti-influenza A H1N1 virus activity in vitro assay.
