Chemotaxis-related factors are expressed abnormally in bone marrow mesenchymal stem cells of multiple myeloma patients.
- Author:
Hui-Jin HU
1
;
Hua LU
;
Xiao-Ming FEI
;
Jun-Xia LI
;
Jian-Yong LI
Author Information
1. Department of Hematology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Bone Marrow Cells;
metabolism;
Cells, Cultured;
Chemokine CCL2;
metabolism;
Chemokine CXCL12;
metabolism;
Chemotactic Factors;
metabolism;
Hepatocyte Growth Factor;
metabolism;
Humans;
Interleukin-8;
metabolism;
Mesenchymal Stromal Cells;
metabolism;
Multiple Myeloma;
metabolism;
RNA, Messenger;
genetics
- From:
Journal of Experimental Hematology
2011;19(1):59-63
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the mRNA expression levels of hepatocyte growth factor (HGF), stromal cell-derived factor-1 (SDF-1), monocyte chemotactic protein-1 (MCP-1) and interleukin-8 (IL-8) in bone marrow mesenchymal stem cells (MSC) from multiple myeloma (MM) patients. The mRNA expression levels of HGF, SDF-1, MCP-1 and IL-8 in bone marrow MSC from 20 newly diagnosed MM patients were detected by real time quantitative RT-PCR and were compared with that in 9 controls. The results indicated that the mean mRNA expression level of HGF was up-regulated in MM patients, as compared with controls (p < 0.01). However, the mean mRNA expression level of SDF-1 mRNA was down-regulated in MM patients, as compared with controls (p < 0.05). There was no significant difference in the mRNA expression levels of MCP-1 and IL-8 between MM and control cohorts (p > 0.05). It is concluded that BM-MSC from MM patients express HGF, SDF-1, MCP-1, IL-8, but these chemotaxis-related factors expression of bone marrow microenvironment cellular component are dysregulated in MM patients, which may result from the interplay between MM cells and MSC.
