A recombination event occurring between HLA-A and -A loci from father's HLA haplotype chromosome.
- Author:
Xiao-Ping HAN
1
;
Jing-Fen SUN
;
Hong-Shi JIN
;
Hong-Yan WANG
;
Li-Li WANG
;
Chun-Ji GAO
;
Li YU
Author Information
1. Department of Hematology, Chinese PLA General Hospital, Beijng 100853, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Alleles;
Fathers;
Female;
Gene Frequency;
HLA-A Antigens;
genetics;
HLA-B Antigens;
genetics;
HLA-C Antigens;
genetics;
HLA-DQ Antigens;
genetics;
HLA-DR Antigens;
genetics;
Haplotypes;
Histocompatibility Testing;
Humans;
Male;
Pedigree;
Recombination, Genetic
- From:
Journal of Experimental Hematology
2011;19(1):180-183
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed investigate the recombination event occurring between HLA-A and-A loci discovered from father's HLA haplotype chromosome in a family. Peripheral blood samples were collected from a family. HLA class I (-A, -B, and -Cw) and II (-DRB1 and -DQB1) alleles were amplified and typed by both low and high resolution PCR with sequence-specific primers (PCR-SSP) and sequence-based typing (SBT). The results showed that 2 haplotypes of the patient were A(*)3001-B(*)1302-DRB1(*)0701 and A(*)3001-B(*)5601-DRB1(*)1454 respectively, those of her father were A(*)3001-B(*)1302-DRB1*0701 and A(*)1101-B(*)5601-DRB1(*)1454. Family analysis demonstrated that the patient's A(*)3001-B(*)1302-DRB1(*)0701 came from her mother and A(*)1101-B(*)5601-DRB1(*)1454 came from her father, but the A of patient was A(*)3001 and B, DR were the same to her father. This showed that the chromosome exchange and recombination event of father's 2 haplotypes occurring between HLA-A and -A loci at meiosis. And recombinate haploid chromosome was completely inherited to his daughter 1. HLA typing and Paternity testing demonstrated that father was the natural father, and the recombination event occurring between HLA-A and -A loci of the daughter 1 with father's HLA haplotype chromosome. It is concluded that the HLA-A/A of father's HLA haplotype chromosome recombination event occurring between HLA-A an-A loci has been found in a family in China, which helps further study on the mechanisms of HLA recombination.
