Protective effect of compound tianpupian (TPP) against H(2)O(2)-induced apoptosis of murine splenic lymphocytes.
- Author:
Ri-Bao WEI
1
;
Yong-Xin WANG
;
Li CAO
;
Li ZHUO
;
Bo FU
;
Ping LI
Author Information
1. Department of Nephrology, Chinese PLA General Hospital, Beijing 100853, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
drug effects;
Caspase 3;
metabolism;
Drugs, Chinese Herbal;
pharmacology;
Grape Seed Extract;
pharmacology;
Hydrogen Peroxide;
adverse effects;
Lymphocyte Count;
Lymphocytes;
cytology;
drug effects;
Mice;
Mice, Inbred ICR;
Proanthocyanidins;
pharmacology;
Rhodiola
- From:
Journal of Experimental Hematology
2011;19(1):211-214
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to explore the protective effect of compound tianpupian (TPP) against (2)O(2)-induced the apoptosis of murine splenic lymphocytes and its mechanism. The cell apoptosis rate was detected by MTT method; the cell apoptosis and mitochondrial membrance potential were detected by flow cytometry (FCM) with Annexi-V/PI double staining and JC-1 staining method, respectively; and caspase 3 relative activity was determined by colorimetry. The results indicated that after treating with (2)O(2), the absorbance value of cultured lymphocytes and the red/green ratio of JC-1 were reduced, and the apoptotic rate and caspase 3 activity were increased, coculture of (2)O(2)-treated cells with compound TPP increased the cell absorbance ratio and red/green rate of JC-1, while reduced the apoptosis rate and caspase 3 activity. It is concluded that compound TPP alleviates intracellular oxidative damages and dose-dependently inhibited apoptosis of murine splenic lymphocytes through reducing mitochondrial membrane potential and inhibiting caspase 3 activity. This suggests that compound TPP is a potential anti-apoptotic agent.
