Detection of Th17/treg cell-associated cytokines in peripheral blood of patients with graft-versus-host disease and its clinical significance.
- Author:
Jing WANG
1
;
Xin-Bing WANG
;
Jian WANG
;
Hui-Lan LIU
;
Liang-Quan GENG
;
Kai-Yang DING
;
Zi-Min SUN
Author Information
1. Department of Hematology, Anhui Medical University, Hefei 230001, Anhui Province, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Case-Control Studies;
Cytokines;
blood;
Female;
Graft vs Host Disease;
blood;
Humans;
Interleukin-10;
blood;
Interleukin-17;
blood;
Interleukin-23;
blood;
Interleukin-6;
blood;
Male;
T-Lymphocytes, Regulatory;
metabolism;
Transforming Growth Factor beta1;
blood;
Young Adult
- From:
Journal of Experimental Hematology
2011;19(2):422-426
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the peripheral levels and clinical significance of Th17/Treg cell-associated cytokines in patients with acute graft versus host disease (aGVHD) or chronic GVHD (cGVHD), blood samples were collected from 39 hematopoietic stem-cell transplantation patients and 20 healthy donors. The patients included 10 patients with aGVHD, 13 patients with cGVHD and 16 patients without evidence of GVHD. Th17/Treg cell-associated cytokines such as IFNγ, IL-4, IL-6, IL-10, TGF-β(1), IL-17 and IL-23 were detected by ELISA. The results showed that the plasma levels of IFN-γ, IL-4, IL-6, IL-17 and IL-23 significantly increased in patients with aGVHD or cGVHD, compared with the patients without clinical signs of GVHD and the healthy donors (p < 0.05), while IL-10 and TGF-β(1) were obviously lower than that of them (p < 0.05). After aGVHD and cGVHD patients were treated effectively, the plasma levels of IL-6, IL-17 and IL-23 were significantly decreased, and IL-10, TGF-β(1) were significantly increased, while the levels of IFN-γ and IL-4 did not markedly change. The TGF-β(1) level were negatively correlated with IL-6 (r = -0.36, p < 0.05), IL-17 (r = -0.51, p < 0.05) and IL-23 (r = -0.44, p < 0.05) respectively, while there were positive correlations between IL-6 and IL-17 (r = 0.62, p < 0.05), IL-6 and IL-23 (r = 0.71, p < 0.05), IL-17 and IL-23 (r = 0.93, p < 0.05). It is concluded that Th17/Treg cell-associated cytokines may play an important role in the development of a/cGVHD, which helps to find novel targets for developing new strategies of GVHD treatment.
