Influence of TLR2 and TLR4 agonists on migration of cord blood CD34(+) cells.
- Author:
Qian-Song CHENG
1
;
Xing-Bing WANG
;
Jian WANG
;
Hui-Lan LIU
;
Liang-Quan GENG
;
Kai-Yang DING
;
Zi-Min SUN
Author Information
1. Department of Hematology, Anhui University Provincial Hospital, Hefei 230001, Anhui Province, China.
- Publication Type:Journal Article
- MeSH:
Antigens, CD34;
blood;
Cell Movement;
drug effects;
Cells, Cultured;
Chemokine CXCL12;
Fetal Blood;
cytology;
immunology;
Humans;
Lipopeptides;
pharmacology;
Lipopolysaccharides;
pharmacology;
Toll-Like Receptor 2;
agonists;
Toll-Like Receptor 4;
agonists
- From:
Journal of Experimental Hematology
2011;19(2):469-472
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the influence of TLR2 and TLR4 agonists on the migration and adhesion activity of umbilical cord blood (UCB) CD34(+) cells and to explore the underlying mechanism. The expression of TLR2 and TLR4 on UCB CD34(+) cells was detected with flow cytometry. The effect of TLR2 agonist (PAM3CSK4) and TLR2 agonist (LPS) on the migration and adhesion ability of UCB CD34(+) cells was evaluated with chemotaxis and adhesion assays. The results indicated that expression levels of TLR2 and TLR4 were (14.2 ± 3.8)%, (19.6 ± 4.1)% respectively. Compared with the control group, the migration activity of UCB CD34(+) cells toward SDF-1 decreased significantly in LPS group (p < 0.01). The adhesion activity was not altered significantly in LPS group. However, both the migration activity towards SDF-1 and the adhesion activity of UCB CD34(+) cells were not changed significantly in PAM3CSK4 group. Further study found that LPS did not affect the expression level of CXCR4 on CD34(+) cells, but could inhibit the spontaneous migration ability of CD34(+) cells. It is concluded that TLR4 activation can decrease the chemotaxis function of CD34(+) cells towards SDF-1, which may associate with the decreased spontaneous migration ability of CD34(+) cells.
