Methylation status of IEX-1 gene promotor CpG island in malignant hematopoietic cell lines.
- Author:
Xiao-Yu LI
1
;
Jian-Zhen SHEN
;
Song-Fei SHEN
;
Hai-Ying FU
;
Hua-Rong ZHOU
;
Dan-Sen WU
;
Yuan-Yuan ZHANG
;
Yong-Qing ZHENG
Author Information
1. Department of Hematology, Fujian Medical University, Fuzhou 350001, Fujian Province, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis Regulatory Proteins;
genetics;
Cell Line, Tumor;
CpG Islands;
DNA Methylation;
Gene Expression Regulation, Neoplastic;
Hematologic Neoplasms;
genetics;
pathology;
Humans;
Leukocytes, Mononuclear;
pathology;
Membrane Proteins;
genetics;
Promoter Regions, Genetic
- From:
Journal of Experimental Hematology
2011;19(2):473-476
- CountryChina
- Language:Chinese
-
Abstract:
This study was to purposed to explore the methylation status changes of IEX-1 gene promoter CpG island and its relevance with occurrence of hematologic malignancies. The methylation status of IEX-1 gene promoter CpG island in 9 NB4, HL-60 U937, Raji, CA46, Jurkat, K562, CEM and Molt4 hematologic malignant cell lines was detected by using methylation-specific PCR, the methylation status of IEX-1 gene promoter CpG island in normal peripheral blood mononuclear cells treated by M. sssI enzyme and the methylation status of IEX-1 gene promoter CpG island in normal peripheral blood mononuclear cells untreated were used as positive and negative controls respectively. The results showed that the hypermethylation of IEX-1 gene promoter CpG islands was detected in NB4, Molt4 and Raji cell lines, as well as in normal peripheral blood mononuclear cells treated by M. sssl enzyme; the partial methylation status was found in CA46, CEM, U937, K562, HL-60 and Jurkat cell lines; the unmethylation status was observed in untreated normal peripheral blood mononuclear cells. It is concluded that the changes of methylation status of gene IEX-1 promoter CpG island correlates with hematologic malignancies to a certain extent.
