Differentiation and malignant suppression induced by mouse erythroid differentiation and denucleation factor on mouse erythroleukemia cells.
- Author:
Han DAISHU
1
;
Zhao QING
;
Ge YEHUA
;
Zhou JIANPING
;
Ma JING
;
Chen KEQUAN
;
Xue SHEPU
Author Information
- Publication Type:Journal Article
- MeSH: Activins; genetics; pharmacology; Animals; Cell Differentiation; drug effects; Cell Division; drug effects; Friend murine leukemia virus; Globins; biosynthesis; genetics; Inhibin-beta Subunits; genetics; pharmacology; Leukemia, Erythroblastic, Acute; metabolism; pathology; Mice; Proto-Oncogene Proteins c-myc; biosynthesis; genetics; RNA, Messenger; biosynthesis; Transfection; Tumor Cells, Cultured
- From: Chinese Medical Sciences Journal 2002;17(4):199-203
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the roles of mouse erythroid differentiation and denucleation factor (MEDDF), a novel factor cloned in our laboratory recently, in erythroid terminal differentiation.
METHODSMouse erythroleukemia (MEL) cells were transfected with eukaryotic expression plasmid pcDNA-MEDDF. Then we investigated the changes on characteristics of cell growth by analyzing cells growth rate, mitotic index and colony-forming rate in semi-solid medium. The expressions of c-myc and beta-globin genes were analysed by semi-quantitative RT-PCR.
RESULTSMEL cells transfected with pcDNA-MEDDF showed significant lower growth rate, mitotic index, and colony-forming rate in semi-solid medium (P<0.01). The percentage of benzidine-positive cells was 32.8% after transfection. The expression of beta-globin in cells transfected with pcDNA-MEDDF was 3.43 times higher than that of control (MEL transfected with blank vector, pcDNA3.1), and the expression of c-myc decreased by 66.3%.
CONCLUSIONSMEDDF can induce differentiation of MEL cell and suppress its malignancy.
