Study on the anti-tumor efficacy induced by heat shock protein 70-peptide complexes derived from tumor cells.
- Author:
Qingguo FU
1
;
Wei ZHANG
;
Fandong MENG
;
Renxuan GUO
;
Zhenyu YAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; CD8 Antigens; blood; Cell Membrane; chemistry; HSP70 Heat-Shock Proteins; biosynthesis; isolation & purification; therapeutic use; Liver Neoplasms, Experimental; chemistry; drug therapy; immunology; pathology; Mice; Neoplasm Transplantation; T-Lymphocytes, Cytotoxic; immunology; Tumor Cells, Cultured
- From: Chinese Medical Sciences Journal 2002;17(3):153-156
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the efficacy and explore the mechanism of the anti-tumor immunity elicited by heat shock protein 70-peptide complexes (HSP70-PC) derived from tumor cells.
METHODSCells culture, flow cytometric analysis, affinity chromatography for protein purification, SDS-PAGE, Western-blotting and animal experiment were used.
RESULTSHSP70-PC immunization rendered protective effect to both naive tumorl-bearing mice. All of the naive mice obtained complete resistance to Hcaf cell attack; 40% of the tumor-bearing mice survived for over 90 days, whereas the mice of control group died within 2 weeks (P < 0.01). CD8+ subset of T lymphocytes in the peripheral blood of immunized mice increased by 12%.
CONCLUSIONHSP70-PC induces anti-tumor immunity via activation of cytotoxic T lymphocytes (CTLs), and it possesses strong tumor vaccine effect. Our research adds more evidence to support the clinical use of HSP70-PC to fight human cancers.
