Expression of rhBMP-7 gene in transduced bone marrow derived stromal cells.
- Author:
Deyu DUAN
1
;
Jingyuan DU
;
Hong WANG
;
Yong LIU
;
Xiaodong GUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Cells; cytology; metabolism; Bone Morphogenetic Protein 7; Bone Morphogenetic Proteins; biosynthesis; genetics; Cells, Cultured; Genetic Vectors; genetics; Rabbits; Recombinant Proteins; biosynthesis; Stromal Cells; cytology; metabolism; Tibia; cytology; Transfection; Transforming Growth Factor beta
- From: Chinese Medical Sciences Journal 2002;17(3):157-159
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the possibility of expression of exogenous gene in transduced bone marrow derived stromal cells (BMSCs).
METHODSThe marker gene, pbLacZ, was transferred into cultured BMSCs and the expression of transduced gene by X-gal staining was examined. Then plasmid pcDNA3-rhBMP7 was delivered to cultured BMSCs. Through immunohistochemical staining and RT-PCR assay, the expression of rhBMP7 gene was detected.
RESULTSThe exogenous gene could be expressed efficiently in transduced BMSCs.
CONCLUSIONThe present study provided a theoretical basis to gene therapy on the problems of bone and cartilage tissue.
