Analysis of N-terminal amino acid sequence of 12 000-protein in gingival crevicular fluid and its clinical significance.
- Author:
Rongji WANG
1
;
Huanxin MENG
;
Zhibin CHEN
;
Caifang CAO
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Electrophoresis, Polyacrylamide Gel; Gingival Crevicular Fluid; chemistry; Humans; Leukocyte L1 Antigen Complex; Periodontium
- From: Chinese Journal of Stomatology 2002;37(4):297-299
- CountryChina
- Language:English
-
Abstract:
OBJECTIVESTo study the essence of N-terminal amino acid sequence of 12 000-protein in gingival crevicular fluid (GCF).
METHODSGCF samples from patients with RPP and AP were collected. 12 000-protein was separated by SDS-PAGE and transformed to PVDF by electronic transformation. The aim band was cut to be analyzed in 491 Protein Sequencer.
RESULTSThe first ten of N-terminal amino acid sequence of 12 000-protein in GCF was Met, Leu, Thr, Glu, Leu, Glu, Lys, Ala, Leu, Asn. Through checking up in MS-Edman, the sequence was similar to "Ca binding protein, MRP8" which is the light subunit of Calprotectin.
CONCLUSIONSCalprotectin is a major protein in granulocytes and monocytes, and is related to many inflammatory diseases, maybe served as a effective marker for evaluating the inflammation of periodontium.
