Cloning of HEV recombinant protein expressed in Pichia pastoris
- Author:
Nga Tuyet Nguyen
- Publication Type:Journal Article
- Keywords:
yeast
- MeSH:
Cloning, Organism;
Proteins
- From:Journal of Preventive Medicine
2000;10(4):46-51
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
The fragment in ORF2 of HEV genome, which is coded for structural protein was transformed into Pichia pastoris GS115 strain (Mut+) after confirmation of positives clones by PCR and DNA sequencing. After induction by methanol for 4 days, shaking at 30oC, the cells were collected and disrupted. The purification of recombinant protein was checked by SDS-PAGE and the presence of HEV recombinant antigen was identified by Western Blot. High antigenicity of HEV recombinant protein was constructed
