Production of a fragment of glycoprotein G of herpes simplex virus type 2 and evaluation of its diagnostic potential.
- Author:
Tao LIU
1
;
Ji Feng LIU
2
;
Hua YU
1
;
Guo Jing SI
1
;
Jun HU
1
;
Jun LI
1
Author Information
- Publication Type:Journal Article
- Keywords: baculovirus expression system; glycoprotein G; herpes simplex virus type 2; immunodominant fragment; indirect enzyme-linked immunosorbent assay
- MeSH: Adult; Antibodies, Viral; blood; Enzyme-Linked Immunosorbent Assay; Female; Herpes Genitalis; diagnosis; virology; Herpes Simplex; diagnosis; virology; Herpesvirus 1, Human; isolation & purification; Herpesvirus 2, Human; isolation & purification; Humans; Immunoglobulin G; chemistry; Male; Polymerase Chain Reaction; Reagent Kits, Diagnostic; Reference Values; Reproducibility of Results; Sensitivity and Specificity; Serologic Tests; methods
- From:Singapore medical journal 2015;56(6):346-352
- CountrySingapore
- Language:English
-
Abstract:
INTRODUCTIONHerpes simplex virus type 2 (HSV-2) is the most common cause of genital herpes. Glycoprotein G (gG) is a prototype antigen for type-specific serodiagnosis distinguishing between HSV type 1 (HSV-1) and HSV-2 infections. As immunological diagnosis kits for accurate differentiation between HSV-1 and HSV-2 antibodies can be expensive, there is a need to develop a convenient, sensitive, specific and cost-effective serodiagnostic kit.
METHODSWe successfully expressed a fragment of gG comprising residues 321-580 of HSV-2 with histidine tag (gG(321-580His)) in a Bac-to-Bac baculovirus expression system, which had an antigenicity similar to its native counterpart. An indirect enzyme-linked immunosorbent assay (ELISA) was developed using gG(321-580His) as the diagnostic antigen and evaluated by comparison with a commercial HerpeSelect 2 ELISA immunoglobulin G kit as reference.
RESULTSIn testing 318 field serum samples, the diagnostic relative sensitivity and specificity of the developed gG(321-580His)-ELISA test in qualitative comparison with the commercial kit were 93.81% and 96.74%, respectively, and the accuracy was 94.65%.
CONCLUSIONThe study indicates that gG(321-580His) has a high diagnostic potential for HSV-2 virus serodiagnosis in humans.
