Expression of mRNA of GATA-3 and T-bet in the peripheral blood mononuclear cells of atopic asthmatics.
- Author:
Young Koo JEE
1
;
Yun Seop KIM
;
Jae Suk PARK
;
Kye Young LEE
Author Information
1. Department of Internal Medicine, College of Medicine, Dankook University, Cheonan, Korea. ykjee@dankook.ac.kr, ykjee@dku.edu
- Publication Type:Original Article
- Keywords:
Asthma;
Atopic;
Transcription factor
- MeSH:
Asthma;
Calcium;
Cell Differentiation;
Cytokines;
Inflammation;
Interleukin-13;
Interleukin-4;
Interleukin-5;
RNA, Messenger*;
Transcription Factors
- From:Korean Journal of Medicine
2004;66(1):67-74
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: It is well known that the expression of Th2 cytokines are up-regulated in the atopic asthma. The study was to investigate the expression of transcription factors, such as GATA-3, c-maf, T-bet which are known to be involved in the T cell differentiation in the peripheral blood mononuclear cells (PBMCs) of atopic asthmatics. METHODS: PBMCs were obtained from non-atopic controls and atopic asthmatics and cultured for 48 hours, and then 12-o-tetracanoylphorbol-13-acetate (PMA) and calcium ionophore (ionomycin) were added. mRNA of GATA-3, c-maf, T-bet, IL-4, IL-5, IL-13 and IFN-gamma were measured by RT-PCR RESULTS: mRNAs of Th2 cytokines, such as IL-4, IL-5 and IL-13 were expressed higher in the atopic asthmatics, and that of Th1 cytokine, IFN-gamma was expressed higher in the non-atopic controls. GATA-3 and c-maf were expressed higher and T-bet was expressed lower in the atopic asthmatics. Expressions of GATA-3, c-maf and T-bet were not changed with the stimulation of PMA/Ionomycin. CONCLUSION: This study suggest that GATA-3, c-maf and T-bet participate in the Th2 type inflammation in atopic asthmatics, like GATA-3 and c-maf as stimulatory factors, and T-bet as inhibitory factor.
