Mineralization of PLGA-ASP-PEG modified with RGD-containing peptide.
- Author:
Yulin SONG
1
;
Qixin ZHENG
;
Jianfeng ZHENG
Author Information
1. Department of Orthopaedics, Second Affiliated Hospital, Nanchang University, Nanchang 330006, China.
- Publication Type:Journal Article
- MeSH:
Biocompatible Materials;
chemistry;
Bone Substitutes;
Bone and Bones;
metabolism;
Calcification, Physiologic;
Lactic Acid;
chemistry;
Oligopeptides;
chemistry;
Osteogenesis;
drug effects;
Peptides;
chemical synthesis;
pharmacology;
Polyglycolic Acid;
chemistry;
Surface Properties
- From:
Journal of Biomedical Engineering
2009;26(5):1056-1059
- CountryChina
- Language:Chinese
-
Abstract:
The RGD-containing peptide was used to modify the surface of porous PLGA-[ASP-PEG], and was incubated in the modified simulated body fluid (mSBF) for two weeks. The mineralization of PLGA-[ASP-PEG] was explored. The active peptide was used to modify PLGA-[ASP-PEG] through cross-linker (Sulfo-LC-SPDP), characterized by X-ray photoelectron spectroscopy (XPS) the peptide-modified PLGA-[ASP-PEG] (Experiment group, EG) and PLGA-[ASP-PEG] without modification (Control group, CG) were all incubated in mSBF for two weeks, confirmed by observation of Scanning electron microscope(SEM) and measurements of Energy dispersive analysis system of X-ray (EDS) and X-ray diffractometry (XRD). XPS indicated that the binding energy of sulphur in EG was 164eV, and the ratio of carbon to sulphur in EG was 99.746 : 0.1014, however, sulphur was not detected in CG; SEM analysis demonstrated that the mineralization layers were more consecutive and compact in EG than in CG. The results of EDS and XRD indicated that the main component of mineral was hydroxyapatite, and the ratio of Ca/P was 1.60 in EG, and 1.52 in CG. RGD-containing peptide provided enough functional groups for mineralization; the mineralized peptide- modified PLGA-[ASP-PEG] possessed the bonelike microstructure.
