The proliferating ability inhibited by energy controllable steep pulse in breast cancer cell line and its mechanism.
- Author:
Yunshan ZHU
1
;
Lina HO
;
Xiaojing DONG
;
Xiaodong LUO
;
Caixin SUN
Author Information
1. Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
radiation effects;
Breast Neoplasms;
pathology;
Cell Line, Tumor;
Cell Proliferation;
radiation effects;
Electric Stimulation Therapy;
methods;
Electromagnetic Fields;
Electroporation;
methods;
Female;
Humans
- From:
Journal of Biomedical Engineering
2009;26(5):1060-1063
- CountryChina
- Language:Chinese
-
Abstract:
This was a study aimed to observe the proliferating ability inhibited by energy controllable steep pulse (ECSP) and to detect the expression of gene with relation to the proliferating ability of the tumor in breast cancer cell line; the possible mechanisms were also addressed. Human breast cancer cell line MDA-MB-231 was treated with ECSP; the apoptosis and the expression of tumor suppressor gene--Rb genes and E2F1 genes in ECSP group and control group were detected by TUNEL staining and Reverse Transcripitional PCR respectively. ECSP was found to inhibit the proliferating ability of breast cancer cells markedly, the cell amount in ECSP group decreased and the TUNEL positive cells increased obviously, compared to control; 24 hours after treatment the expression of Rb genes mRNA increased, whereas the expression of E2F1 mRNA decreased. These findings indicate that the proliferating ability of breast cancer cells can be inhibited by ECSP markedly, the apoptosis of breast cancer cell can be induced by ECSP, and the Rb genes and E2F1 genes may be involved in the course.
