Study on morphology of surface atoms conformation of nanogold-based genechip.
- Author:
Dayong GU
1
;
Bing LIANG
;
Huawei YU
;
Weiping LU
;
Yuanguo ZHOU
;
Ya'ou ZHANG
Author Information
1. Research Institute of Disease Control and Prevention, Shenzhen Entry-exit Inspection and Quarantine, Shenzhen 518045, China.
- Publication Type:Journal Article
- MeSH:
Gold;
Metal Nanoparticles;
chemistry;
Microscopy, Atomic Force;
methods;
Microscopy, Scanning Tunneling;
methods;
Molecular Conformation;
Nanotechnology;
methods;
Oligonucleotide Array Sequence Analysis;
instrumentation;
methods;
Particle Size;
Surface Properties
- From:
Journal of Biomedical Engineering
2009;26(6):1214-1217
- CountryChina
- Language:Chinese
-
Abstract:
Conformations of surface atoms in various stages of nanogold-based genechip testing were scanned by the atomic force microscope based on the scanning tunneling microscope. The findings were: First, the surface atoms of genechip slide (formylphenyl glass) were in a regular porous-arrangement; Second, after combination with probe, the regular porous arrangement changed to be irregular; Third, after hybridization with the target nucleic acid, the surface atoms were once again in a cable-like arrangement which was relatively structured and intensively cross-parallel. However, after the silver staining, the surface atoms showed a larger block structure with serious unevenness. From these results we can intuitively know the process and differences in probe combination, nucleic acid hybridization, and silver staining. Moreover, the relevant experiment was verified at the micro-level.
