Relationship between regulation effect of salvia miltiorrhiza on AQP2 in kidney and promoting blood circulation and diuresis.
- Author:
Xiao-Jing DONG
;
Liang-Feng GUO
;
Rui YAO
;
Song-Yan XUE
;
Feng LI
- Publication Type:Journal Article
- MeSH:
Animals;
Aquaporin 2;
genetics;
metabolism;
Blood Circulation;
drug effects;
Diuresis;
drug effects;
Drugs, Chinese Herbal;
administration & dosage;
Humans;
Kidney;
blood supply;
drug effects;
metabolism;
physiopathology;
Kidney Diseases;
drug therapy;
genetics;
metabolism;
physiopathology;
Male;
Rats;
Salvia miltiorrhiza;
chemistry
- From:
China Journal of Chinese Materia Medica
2014;39(16):3162-3165
- CountryChina
- Language:Chinese
-
Abstract:
Partial nature of "promoting blood circulation and dieresis" of Salvia Miltiorrhizain was initially demonstrated by investigating the regulation effect of AQP2 expression in kidney of trauma blood stasis model rats with the Salvia Miltiorrhizain so as to provide guidance for its clinical deployment of administration. Random allocation was taken to averagely divide 30 SD rats into two groups: 10 rats in normal group and 20 rats in blood stasis syndrome group. Trauma blood stasis rat model was established by quantitatively beating. Then the rat model group was divided into model group and salvia group. After 7 days of treatment, the rat kidney AQP2 expression was detected, the content of urine AQP2 was compared and the damaged local muscle and kidney pathological changes were observed by immunohistochemical method and western blot method. Compared with that of the normal group, rats in model group had inflammatory cells infiltration, blood stasis and edema of the injured local muscles and up-regulated AQP2 expression, decreasing urinary output, and kidney tissues blood stasis and edema (P < 0.05). On the other hand, compared with that of the model group, those parameters of rats in salvia group were all decreasing except urine output (P < 0.05). Such result indicated that Salvia Miltiorrhiza can reduce trauma blood stasis rat content of urine AQP2 and down-regulated AQP2 expression in kidney tissue, so as to reduce the reabsorption of water by renal tubular and increase urine output. The promoting blood circulation effect of Salvia Miltiorrhizain can alleviate the degree of the damaged tissue edema and encourage urine drainage. This therapy is closely related to the effect of regulating AQP2 in kidney by salvia, so the purpose of this study by verifying "promoting blood circulation and diuresis" as the mechanism for the regulation effect of the salvia on AQP2 expression.
