Simultaneous quantitation of artemisinin, arteannuin B, artemisic acid, and scopoletin in mice plasma by HPLC-MS.
- Author:
Qing MIN
;
Wei LU
;
Man-Yuan WANG
;
Dong ZHANG
;
Tian-Yan ZHOU
;
Liang LI
- Publication Type:Journal Article
- MeSH:
Animals;
Artemisinins;
blood;
pharmacokinetics;
Chromatography, High Pressure Liquid;
instrumentation;
methods;
Dose-Response Relationship, Drug;
Male;
Mice;
Reproducibility of Results;
Scopoletin;
blood;
pharmacokinetics;
Spectrometry, Mass, Electrospray Ionization;
methods
- From:
China Journal of Chinese Materia Medica
2014;39(17):3306-3310
- CountryChina
- Language:Chinese
-
Abstract:
The objective of this study is to develop a sensitive and reliable high-performance liquid chromatography mass spectrometry (LC-MS) method for simultaneous determination of artemisinin, arteannuin B, artemisic acid, and scopoletin, and study the pharmacokinetics of the four constituents in mouse serum after oral administration of the four components to mice. The analytical column used was Agilent Zorbax SB-C18 (2.1 mm x 150 mm, 5 mm). The mobile phase was acetonitrile: 0.5% acetic acid (60: 40) and the flow rate was 0.3 mL x min(-1). The temperature of the column was 40.0 degrees C. In this condition, we established an analysis method to simultaneously determine the four components. A sensitive and specific liquid chromatography-mass spectrometric (LC-MS) method was developed and validated for the determination of artemisin in derivatives in mice plasma. The method we established has a linear range of 5-3 000 μg x L(-1) with a good sensitivity and specificity for all of the four components. This method is simple, rapid, accurate and suitable for the determination of the content of the four compounds.
