Effect of andrographolide on Candida albicans biofilm dispersion.
- Author:
Gao-Xiang SHI
;
Yuan-Yuan YAN
;
Jing SHAO
;
Ke-Qiao LU
;
Meng-Xiang ZHANG
;
Tian-Ming WANG
;
Chang-Zhong WANG
- Publication Type:Journal Article
- MeSH:
Anti-Inflammatory Agents;
pharmacology;
Biofilms;
drug effects;
Candida albicans;
genetics;
physiology;
ultrastructure;
Diterpenes;
pharmacology;
Dose-Response Relationship, Drug;
Fungal Proteins;
genetics;
Gene Expression Regulation, Fungal;
drug effects;
HSP90 Heat-Shock Proteins;
genetics;
Microscopy, Electron, Scanning;
Reverse Transcriptase Polymerase Chain Reaction;
Time Factors
- From:
China Journal of Chinese Materia Medica
2014;39(17):3339-3343
- CountryChina
- Language:Chinese
-
Abstract:
Along with the increase in fungal infections, Candida albicans prevention and control become the focus of anti-fungal infection at present. This study aims to discuss the effect monomer andrographolide (AG) on C. albicans biofilm dispersion. In the experiment, micro-well plates and medical catheter pieces were used to establish the C. albicans biofilm model. It was discovered by XTT assay and flat band method that 1 000, 500, 250 mg x L(-1) AG could impact the activity of C. albicans biofilm dispersion cells. The morphological structures of residual biofilms on catheter pieces were observed with scanning electron microscopy, which showed that 1 000, 500, 250 mg x L(-1) AG could induce C. albicans biofilm dispersion in a dose-dependent manner, and the dispersed cells were dominated by the yeast phase. According to the real-time fluorescence quantification PCR (qRT-PCR) test, AG could up-regulate HSP90 expression and down-regulate UME6 and PES1 expressions. This study demonstrates that AG could induce C. albicans biofilm dispersion to some extent.
