Effect of carnosol against proliferative activity of breast cancer cells and its estrogen receptor subtype's mediation and regulation mechanisms.
- Author:
Pi-Wen ZHAO
;
David Yue-Wei LEE
;
Zhong-Ze MA
;
Yan-Ling SUN
;
Shi-Ying TAO
;
Jin-Feng ZANG
;
Jian-Zhao NIU
- Publication Type:Journal Article
- MeSH:
Blotting, Western;
Breast Neoplasms;
metabolism;
pathology;
Cell Cycle;
drug effects;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Diterpenes, Abietane;
chemistry;
pharmacology;
Dose-Response Relationship, Drug;
Estrogen Receptor Modulators;
pharmacology;
Estrogen Receptor alpha;
antagonists & inhibitors;
metabolism;
Estrogen Receptor beta;
antagonists & inhibitors;
metabolism;
Female;
Flow Cytometry;
Humans;
Molecular Structure;
Pyrazoles;
pharmacology;
Pyrimidines;
pharmacology
- From:
China Journal of Chinese Materia Medica
2014;39(17):3344-3348
- CountryChina
- Language:Chinese
-
Abstract:
Carnosol has been proved to have anti-breast cancer effect in previous research. But its ER subtype's specific regulation and mediation mechanisms remain unclear. The aim of this study is to observe the effect of carnosol on cell proliferation and its estrogen receptor α and β's specific regulation and mediation mechanisms with ER positive breast cancer T47D cell. With estrogen receptor α and β antagonists MPP and PHTPP as tools, the MTT cell proliferation assay was performed to observe the effect of carnosol on T47D cell proliferation. The changes in the T47D cell proliferation cycle were detected by flow cytometry. The effect of carnosol on ERα and ERβ expressions of T47D cells was measured by Western blot. The findings showed that 1 x 10(-5)-1 x 10(-7) mol x L(-1) carnosol could significantly inhibit the T47D cell proliferation, which could be enhanced by MPP or weakened by PHTPP. Meanwhile, 1 x 10(-5) mol x L(-1) or 1 x 10(-6) mol x L(-1) carnosol could significantly increase ERα and ERβ expressions of T47D cells, and remarkably increase ERα/ERβ ratio. The results showed that carnosol showed the inhibitory effect on the proliferation of ER positive breast cancer cells through target cell ER, especially ERβ pathway. In the meantime, carnosol could regulate expressions and proportions of target cell ER subtype ERα and ERβ.
