Study on impact of ethanol extracts from Sedum sarmentosum in inhibiting STAT-3 signaling and inducing apoptosis of human hepatocellular carcinoma cell line HepG2.
- Author:
Jun-Ying ZENG
;
Sheng-Hua LI
;
Xian-Jin WU
;
Dan LIU
;
Xiong WAN
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Blotting, Western; Caspase 3; metabolism; Caspase 9; metabolism; Cell Proliferation; drug effects; Dose-Response Relationship, Drug; Ethanol; chemistry; Flow Cytometry; Hep G2 Cells; Humans; Myeloid Cell Leukemia Sequence 1 Protein; metabolism; Plant Extracts; chemistry; pharmacology; Poly(ADP-ribose) Polymerases; metabolism; Proto-Oncogene Proteins c-bcl-2; metabolism; STAT3 Transcription Factor; metabolism; Sedum; chemistry; Signal Transduction; drug effects; Time Factors
- From: China Journal of Chinese Materia Medica 2014;39(17):3349-3352
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the impact of ethanol extracts from Sedum sarmentosum (ESB) on STAT-3 signaling and its probable molecular mechanism in inducing apoptosis.
METHODMTT assay was used to detect the impact of ESB on HepG2 cell proliferation. FITC-Annexin V-FITC /PI double-labeling were used to investigate the impact on hepatoma carcinoma cell apoptosis. Western blot analysis was used to test the expression levels of cell apoptosis-related proteins Caspase-3, Caspase-9, PARP, P-STAT-3 (Tyr705) , STAT-3, Bcl-2, Mcl-1.
RESULTESB could notably inhibit proliferation of HepG2 cells, and induce HepG2 cell apoptosis, with the dose-dependent inhibitory effect. In addition, ESB could inhibit STAT-3 signaling, down-regulate Mcl-1 and Bcl-2 expressions, and induce degradation/activation of apoptosis-related proteins Caspase-3 and Caspase-9 and PARP degradation in a dose-dependent manner.
CONCLUSIONESB inhibits HepG2 cell proliferation and induces apoptosis by inhibiting STAT-3 signaling and Mcl-1 and Bcl-2 expressions.