A preliminary genetic reassortment between Hantaan virus and Seoul virus strains.
- Author:
Wenzhen KANG
1
;
Changxing HUANG
;
Xuefan BAI
;
Weisong YANG
;
Guangyu LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cercopithecus aethiops; Genome, Viral; Genotype; Hantaan virus; genetics; RNA, Viral; genetics; Reassortant Viruses; genetics; Reverse Transcriptase Polymerase Chain Reaction; Seoul virus; genetics; Vero Cells
- From: Chinese Journal of Epidemiology 2002;23(1):46-49
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the frequency and characteristics of reassortment among Hantaan and Seoul viruses causing hemorrhagic fever with renal syndrome (HFRS).
METHODSMixed infections were initiated in tissue culture, using Hantaan virus strain 76 - 118 and Seoul virus strain SR-11. Potential reassortant virus plaques were picked out by multiplex RT-PCR, using primers specific for individual genome segments (L, M, S) of each strain.
RESULTSMost of the progeny virus plaques (68.19% of 44) had parental genotype of 76 - 118 strain or SR-11 strain while 2 of 44 plaques had mixed genotypes that yielded RT-PCR bands for the same segment of both parental strains. Reassortant viruses were detected in 68.19% of 44 progeny plaques tested, involving the M and S segments. In addition, approximately 4.55% of the progeny virus plaques appeared to contain S or M segments originating from both parental virus strains, showing that they were diploid.
CONCLUSIONGenetic reassortment can occur between Hantaan virus and Seoul virus strains.
