Detection and sequential analysis of Granulocytic ehrlichia 444-Epank gene.
- Author:
Qiumin ZHAO
1
;
Wuchun CAO
;
Jianmin LI
;
Panhe ZHANG
;
Shanhu CHEN
;
Kexin CAO
;
Dongqi GAO
;
Hong YANG
;
Xitan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: DNA, Bacterial; analysis; Ehrlichia; classification; genetics; isolation & purification; Ehrlichiosis; microbiology; Genes, Bacterial; Humans; Phylogeny; Polymerase Chain Reaction; Sequence Analysis, DNA
- From: Chinese Journal of Epidemiology 2002;23(4):286-288
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo provide further pathogenic evidence of Granulocytic ehrlichia infection in China.
METHODSSpecific primers derived from 444-Epank gene were used to amplify Granulocytic ehrlichia DNA from specimens of ticks, animals and human blood. PCR products of ticks were cloned and sequenced.
RESULTS444 bp specific DNA fragments were amplified from 2 of 62 pools of Ixodes persulcatus collected from Heilongjiang province and 1 of 129 blood specimens from forest workers in Inner Mongolia. Eight animal specimens were negative. PCR products from ticks were then cloned and sequenced. It differed at 23 positions in comparison to American strain (AF047897) with 94.9% homology. The homology of deduced ammonia was 88.44%.
CONCLUSIONOur findings further confirmed that Granulocytic ehrlichia infection did exist in China.
