A study on genetic polymorphism of rRNA gene pattern of Vibrio cholerae O139 in China.
- Author:
Mei QU
1
;
Biao KAN
;
Guoming QI
;
Yanqing LIU
;
Shouyi GAO
Author Information
- Publication Type:Journal Article
- MeSH: Phylogeny; Polymorphism, Genetic; RNA, Ribosomal, 16S; genetics; RNA, Ribosomal, 23S; genetics; Ribotyping; Species Specificity; Vibrio cholerae O139; classification; genetics
- From:Chinese Journal of Epidemiology 2002;23(3):203-205
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigated the genetic polymorphism of the isolated strains using ribotyping method.
METHODSOne hundred twenty-two strains of V. cholerae O139 isolated from different areas of China from 1993 to 1999 were selected and characterized with ribotyping, including 16s rDNA and 23s rDNA probes.
RESULTSOne hundred twenty-two strains were differentiated into 10 different ribotypes (RT1-RT10) on the basis of rRNA gene probes hybridization (with Bgl I digestion), which consisted of 7 - 9 bands between 12 and 1.5 kb in size. RT1 and RT3, as two predominant ribotypes, comprised most number of the strains which spread to the extensive range. Nine strains, which are negative to ctxAB, zot and RS individually, belong to 4 special ribotypes. The dendrogram revealing genetic relationship among different clones of V. cholerae O139 showed that the clones belonging to RT1 and RT2 had genetic similarity on high degree, although they were isolated from different regions. The two predominant ribotypes (RT1 and RT3) were distant in genetic relationship.
CONCLUSIONResults showed the clonal diversity and the wide area distribution of V. cholerae O139 strains in China, suggesting the multiple origins of O139 epidemics.
