Analysis on the aetiology of influenza in children in Tianjin.
- Author:
Xiao-hua GUO
1
;
Wei-ping DUAN
;
Shi-rong WANG
;
Hua ZHANG
;
Ying LI
;
Jin-ying CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Child; Child, Preschool; China; epidemiology; Female; Genetic Variation; Hemagglutination Inhibition Tests; Hemagglutination Tests; Humans; Infant; Infant, Newborn; Influenza A virus; classification; isolation & purification; Influenza B virus; classification; isolation & purification; Influenza, Human; epidemiology; etiology; virology; Male
- From: Chinese Journal of Epidemiology 2003;24(1):19-21
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyse the pathogen of child patients with influenza in Tianjin area.
METHODSThe influenza virus isolation was performed by MDCK cells and embryonated eggs. The identification of the isolates was carried out with hemagglutination (HA) and hemagglutination inhibition (HI) tests.
RESULTSTwo hundred and thirty-eight throat swab specimens from children with influenza-like illness were collected in Tianjin area from Oct. 2001 to Mar. 2002 and 64 strains (26.9%) of influenza virus were isolated. Data showed that there were 42 strains (65.6%) of A (H3N2) subtype, 13 strains (20.3%) of A (H1N1) subtype and 9 strains (14.1%) of B type in these positive isolates. All the isolated viruses grew very well in MDCK cells and hemagglutinated with human "O" red blood cells, and most (62/64 strains) of them were able to multiply in embryonated chick eggs. However, there were only 3 isolates with HA positive in inoculating embryonated eggs with the specimens. Meanwhile, it was revealed that out of 55 strains of A type viruses, 53 strains (96.4%) were from O to D phase, 2 strain of A (H3N2) were D phase characters and all B type isolated viruses being D phase properties.
CONCLUSIONThere were three endemic types of influenza viruses-A (H3N2), A (H1N1) and B type in Tianjin area, with A, the main type.
