Gradual algorithm of postconditioning reduced reperfusion injury through mitochondrion pathway in rats
10.3760/cma.j.issn.0253-3758.2010.06.013
- VernacularTitle:后适应渐进模式通过线粒体途径减轻大鼠心肌缺血再灌注损伤
- Author:
Guo-Ming ZHANG
1
;
Tu WANG
;
Tian-De LI
;
Da-Wei ZHANG
;
Xiu-Hua LIU
;
Fei-Fei YANG
;
Hui ZHANG
;
Yu SUN
;
Liang ZHANG
Author Information
1. 解放军总医院
- Keywords:
Myocardial reperfusion injury;
Mitochondria;
Postconditioning
- From:
Chinese Journal of Cardiology
2010;38(6):539-544
- CountryChina
- Language:Chinese
-
Abstract:
Objective The effects of various postconditioning algorithm on reperfusion injury and the role of mitochondrion pathway were investigated in a rat model of reperfusion/injury. Methods Rats were divided into 5 groups : sham, reperfusion/injury ( R/I group), reverse algorithm of postconditioning ( R-Post, 30/10-25/15-15/25-10/30 s of reperfusion/re-occlusion), standard algorithm of postconditioning (S-Post, 4 cycles of 20/20 s of reperfusion/re-occlusion), and gradual algorithm of postconditioning (G-Post, 10/30-15/25-25/15-30/10 s of reperfusion/re-occlusion). Results The levels of Bax,Cytochrome-c, Caspase-9, serum marker of myocardium and apoptosis index were significantly lower while the level of Bcl-2 was significantly higher in the three postconditioning groups than those in R/I group ( all P <0. 05). The levels of Bax (0. 35 ± 0. 10 vs. O. 50 ± 0. 02, P < 0. 05), Cytochrome-c (0. 66 ± 0. 16vs. 1.68 ±0.22, P <0.05), Caspase-9 (0.61 ±0. 17 vs. 1.66 ±0.55, P <0.05), serum marker of myocardium[CK: (251.00 ±45. 16)U/L vs. (388.56 ±75.01) U/L, P<0.05; CK-MB: (146.00 ±60. 12) U/L vs. (291.16 ± 52. 41 ) U/L, P < 0. 05] and apoptosis index [(4. 32 ± 1.16) % vs. ( 8.58 ±1.12 ) %, P < 0. 05] were all significantly lower while Bcl-2 level (2. 00 ± 0. 34 vs. 1.40 ± 0. 18, P < 0. 05 )was significantly higher in G-Post group than those in S-Post group. Moreover, above mentioned cardiac protective effects were significantly stronger in the G-Post group compared to R-Post group ( all P < 0. 05 ).Conclusion In conclusion, gradual algorithm of postconditioning could attenuate reperfusion injury more significantly than standard algorithm, and mitochondrion pathway plays an important role in this cardioprotective process.
