miRNA changes in the reverse remodeling heart of rats.
- Author:
Jue WANG
1
;
Zhe ZHENG
;
Fu-Qian LIN
;
Cheng-Chao SUN
;
Rui-Xia XU
;
Sheng-Shou HU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Collagen; metabolism; Heart Ventricles; metabolism; Male; MicroRNAs; metabolism; Myocytes, Cardiac; metabolism; Rats; Rats, Inbred Lew; Transforming Growth Factor beta; metabolism; Ventricular Remodeling
- From: Chinese Journal of Cardiology 2010;38(8):745-750
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a reverse remodeling heart model in rats and observe collagen and TGF-β expression and relevant microRNAs changes during reverse remodeling.
METHODSLewis rats were divided into four groups including sham (NL, n = 10), abdominal aortic constriction (AAC, n = 10), heterotopic transplantation of abdominal aortic constriction (AAC-HT, n = 9) and heterotopic transplantation of normal heart (HT, n = 8). Left ventricular wall thickness and LV cavity were measured by echocardiography. The cardiomyocyte cross-sectional area (CSA) was determined on HE stained sections. Immunohistochemical and qRT-PCR were used to detect collagen and TGF-β expressions. miRNAs were detected by MicroRNA microarray.
RESULTSHeart weight, left ventricular wall thickness and CSA were significantly increased in AAC hearts compared to those in the NL and AAC-HT hearts. The collagen and TGF-β were increased in AAC hearts and further increased in AAC-HT hearts. miRNA microarray evidenced more than two folds changes on 82 miRNAs compared to NL (10 in AAC, 32 in AAC-HT and 40 in HT).
CONCLUSIONRat abdominal aortic constriction and heterotopic transplantation could be used as a reverse remodeling heart model and significant collagen and TGF-β as well microRNA expression changes were evidenced in this model.