miRNA changes in the reverse remodeling heart of rats
10.3760/cma.j.issn.0253-3758.2010.08.015
- VernacularTitle:微小RNA在大鼠心脏逆重构中的变化
- Author:
Jue WANG
1
;
Zhe ZHENG
;
Fu-Qiang LIN
;
Cheng-Chao SUN
;
Rui-Xia XU
;
Sheng-Shou HU
Author Information
1. 北京协和医学院中国医学科学院阜外心血管病医院
- Keywords:
Ventricular remodeling;
MicroRNAs;
Collagen;
Transforming growth factor beta
- From:
Chinese Journal of Cardiology
2010;38(8):745-750
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a reverse remodeling heart model in rats and observe collagen and TGF-β expression and relevant rmicroRNAs changes during reverse remodeling. Methods Lewis rats were divided into four groups including sham(NL, n = 10), abdominal aortic constriction(AAC, n = 10),heterotopic transplantation of abdominal aortic constriction(AAC-HT, n = 9)and heterotopic transplantation of normal heart(HT, n = 8). Left ventricular wall thickness and LV cavity were measured by echocardiography. The cardiomyocyte cross-sectional area(CSA)was determined on HE stained sections.Immunohistochemical and qRT-PCR were used to detect collagen and TGF-β expressions. miRNAs were detected by MicroRNA microarray. Results Heart weight, left ventricular wall thickness and CSA were significantly increased in AAC hearts compared to those in the NL and AAC-HT hearts. The collagen and TGF-β were increased in AAC hearts and further increased in AAC-HT hearts. miRNA microarray evidenced more than two folds changes on 82 miRNAs compared to NL(10 in AAC, 32 in AAC-HT and 40 in HT).Conclusion Rat abdominal aortic constriction and heterotopic transplantation could be used as a reverse remodeling heart model and significant collagen and TGF-β as well microRNA expression changes were evidenced in this model.
