Effects of telmisartan on 4-Aminopyridine-sensitive voltage dependant potassium channel of lymphocyte derived from spontaneously hypertensive rat
10.3760/cma.j.issn.0253-3758.2010.08.016
- VernacularTitle:自发性高血压大鼠淋巴细胞4-氨基吡啶敏感Kv通道电流变化及替米沙坦对其阻滞作用
- Author:
Jian LUO
1
;
Ke-Tao MA
;
Yuan-Ming ZHANG
;
Jun-Qiang SI
;
Ping LIANG
;
Jing LI
Author Information
1. 新疆医科大学第一附属医院
- Keywords:
Telmisartan;
Hypertension;
Potassium channels;
Lymphocytes
- From:
Chinese Journal of Cardiology
2010;38(8):751-754
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of telmisartan on voltage dependant potassium channel (Kv)expression in lymphocytes from spontaneously hypertensive rat(SHR). Methods Peripheral blood was collected from male SHR aged 16 and 4 weeks. Peripheral lymphocytes were separated from heparinized whole blood by standard Ficoll-Hypaque density gradient centrifugation. The whole-cell Kv currents were recorded with patch-clamp technique in the absence and presence of telmisartan(10,30,100 μmol/L).Real-time PCR was used to determine Kv1.3 mRNA expression in lymphocytes. Results(1)The currents density of Kv was higher in lymphocytes from 16 weeks-old SHR[(119.0 ±9.6)pA/pF]than from 4 weeks-old SHR[(59.0±7.2)pA/pF, P＜0.05].(2)Currents density was positively correlated with systolic blood pressure in 16 weeks-old SHR(r= 0.837,P＜0.05).(3)The lymphocytes Ky 1.3 mRNA expression was significantly higher in 16-weeks-old SHR than in 4-weeks-old SHR(P ＜ 0. 05).(4)Telmisartan reduced the whole-cell Ky currents in a concentration-dependent manner(10.5 ± 3.4)% at 10μmol/L,(45.8 ±3.7)% at 30 μmol/L and(81.6 ±4.2)% at 100 μmol/L, P＜0.01. Conclusions The lymphocyte Kv channel is upregulated in 16 weeks-old SHR suggesting a role of Kv in the pathophysiology of hypertension. Ky current in lymphocyte could be significantly blocked by telmisartan in a concentration dependent manner.
