Effects of a recombinant adenovirus expressing human hypoxia-inducible factor 1α double-mutant on the in vitro differentiation of bone marrow mesenchymal stem cells to cardiomyocytes.
- Author:
Jiao-jie XUE
1
;
Ye-song WANG
;
Hong MA
;
Yuan HU
;
Kang-lin CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Animals; Bone Marrow Cells; cytology; drug effects; metabolism; Cell Differentiation; drug effects; genetics; Cells, Cultured; Genetic Vectors; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; genetics; Male; Mesenchymal Stromal Cells; cytology; drug effects; metabolism; Myocytes, Cardiac; cytology; metabolism; Rats; Rats, Sprague-Dawley; Signal Transduction; Smad4 Protein; metabolism; Transforming Growth Factor beta1; metabolism
- From: Chinese Journal of Cardiology 2010;38(7):638-643
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of mutant hypoxia-inducible factor-1α (HIF-1α) adenovirus (Adeno-HIF-1α-Ala402-Ala564) on cardiomyocytes (CMCs) differentiation from the mesenchymal stem cells (MSCs) co-cultured with CMCs.
METHODSFollowing groups were studied: HIF-1α group (MSCs + CMCs + Ad-HIF-1α), LacZ group (MSCs + CMCs + Ad-LacZ), Sham group (MSCs + CMCs + PBS) and MSC + HIF-1α Group (MSCs + Ad-HIF-1α). MSCs were co-cultured with myocardial cells in proportion of MSCs:CMCs 1:2, after 24 hours, cells were infect with virus (MOI = 100) or treated with PBS, cardiac troponin (cTnT) expression in MSCs was detected 7 days post infection by immunochemical analysis, mRNA expression of HIF-1α, TGF-β(1), Smad4, NKx2.5, GATA-4 was also detected by RT-PCR.
RESULTSHIF-1α increased MSCs differentiation to myocardial cells (differentiation rate 32.68% ± 6.52% vs. 8.28% ± 0.09% in the LacZ group and 10.25% ± 2.20% in the Sham group and 0.32% ± 0.05% in the MSC group (all P < 0.05 vs. HIF-1α group). mRNA expression of HIF, TGF-β(1), Smad4, NKx2.5 and GATA-4 was also significantly upregulated in HIF-1α group all P < 0.05 vs. Sham group).
CONCLUSIONHIF-1α promoted MSCs, co-cultured with myocardial cells, differentiating to cardiomyocytes via upregulating TGF-β(1)/Smad4 signaling pathway.
