Rosuvastatin enhances the protective effects of ischemic postconditioning on myocardial ischaemia-reperfusion injury in type 2 diabetic rat.
- Author:
Wei CAI
1
;
Jun FANG
;
Zhao-yang CHEN
;
Yun-ling LIN
;
Li-ming WU
;
Liang-long CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Diabetes Mellitus, Experimental; complications; Diabetes Mellitus, Type 2; complications; Fluorobenzenes; pharmacology; Hydroxymethylglutaryl-CoA Reductase Inhibitors; pharmacology; Ischemic Postconditioning; Male; Myocardial Reperfusion Injury; prevention & control; Myocardium; pathology; ultrastructure; Nitric Oxide Synthase Type III; metabolism; Phosphatidylinositol 3-Kinases; metabolism; Phosphorylation; Proto-Oncogene Proteins c-akt; metabolism; Pyrimidines; pharmacology; Rats; Rats, Wistar; Rosuvastatin Calcium; Signal Transduction; Sulfonamides; pharmacology
- From: Chinese Journal of Cardiology 2010;38(9):814-818
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEto investigate the combined effect of rosuvastatin (RSV) and ischemic postconditioning (PC) on myocardial ischemia-reperfusion (I/R) injury in a type 2 diabetic rat model.
METHODStype 2 diabetic (induced by streptozotocin plus nicotinamide) rats, undergoing 30 min ischemia and 120 min reperfusion, were divided into six groups (n = 10 each): Sham, I/R without other interventions, RSV before reperfusion, PC with 3 cycles of 10 s reperfusion and 10 s ischemia, RSV + PC and RSV + PC + PI3-K inhibitor LY294002. Myocardial infarct size (IS), ultrastructural change and myocardial expression of phosphorylated eNOS/total eNOS were determined.
RESULTSIS and ultrastructural damages were all significantly reduced and myocardial eNOS phosphorylation was significantly increased in RSV and PC groups compared with the I/R group (all P < 0.05) these beneficial effects were further enhanced by RSV + PC (all P < 0.05 vs. RSV and PC, respectively). The beneficial effects were significantly attenuated by PI3K inhibitor LY294002.
CONCLUSIONSthe results indicate that RSV + PC could alleviate myocardial ischemia-reperfusion injury in this type 2 diabetic model by activating PI3K/AKT/eNOS signaling pathway.
