Effect of ischemia preconditioning on pro- and anti- angiogenic molecule expression and functional arteries formation.
- Author:
Yong WANG
1
;
Cong-xin HUANG
;
Jin-song CHENG
;
Hui WANG
;
Wen-jing WU
;
Wen-qiang LIAO
;
Jian-yan WEN
;
Yuan-nan KE
;
Jin-gang ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: ADAM Proteins; metabolism; ADAMTS1 Protein; Animals; Arteries; metabolism; pathology; Ischemic Preconditioning; Male; Neovascularization, Physiologic; Proto-Oncogene Proteins c-sis; metabolism; Rats; Rats, Sprague-Dawley; Up-Regulation; Vascular Endothelial Growth Factor A; metabolism
- From: Chinese Journal of Cardiology 2010;38(9):819-822
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEto observe the effect of ischemia preconditioning (IPC) on the expression of pro-angiogenic VEGF, PDGF and anti-angiogenic ADAMTS-1, and arteriogenesis.
METHODSrat heart IPC model was made by 4 circles of occluding the LAD for 6 min followed by 6 min of reperfusion. The expression of VEGF, PDGF-B and ADAMTS-1 in the ischemic area was examined with immunohistochemistry at 6, 12 and 24 h after IPC. IPC plus myocardial infarction model was induced by LAD ligation 24 h after IPC, 14 days later, the anti-SM-α-actin antibody was used to detect the mature neovascularization in the border of the infracted area.
RESULTSVEGF, PDGF-B and ADAMTS-1 were significantly upregulated in the ischemic area in IPC group compared with the control group (P < 0.05). Density of mature arteries was also significantly increased in IPC plus MI group than that in MI group (P < 0.05).
CONCLUSIONIPC promoted the formation of mature new arteries which may be modulated by upregulating VEGF, PDGF-B, and ADAMTS-1 expressions.
