Effect of ischemia preconditioning on pro- and anti-angiogenic molecule expression and functional arteries formation
10.3760/cma.j.issn.0253-3758.2010.09.014
- VernacularTitle:缺血预处理对大鼠血管生长物质表达及新生动脉形成的影响
- Author:
Yong WANG
1
;
Cong-Xin HUANG
;
Jin-Song CHENG
;
Hui WANG
;
Wen-Jing WU
;
Wen-Qiang LIAO
;
Jian-Yan WEN
;
Yuan-Nan KE
;
Jin-Gang ZHENG
Author Information
1. 武汉大学人民医院
- Keywords:
Ischemic preconditioning,myocardial;
Neovascularization,physiologic;
Vascular endothelial factors;
Proto-oncogene proteins c-sis;
ADAMTS-1
- From:
Chinese Journal of Cardiology
2010;38(9):819-822
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of ischemia preconditioning (IPC) on the expression of pro-angiogenic VEGF, PDGF and anti-angiogenic ADAMTS-1, and arteriogenesis. Methods Rat heart IPC model was made by 4 circles of occluding the LAD for 6 min followed by 6 min of reperfusion. The expression of VEGF, PDGF-B and ADAMTS-1 in the ischemic area was examined with immunohistochemistry at 6, 12 and 24 h after IPC. IPC plus myocardial infarction model was induced by LAD ligation 24 h after IPC, 14 days later, the anti-SM-α-actin antibody was used to detect the mature neovascularization in the border of the infracted area. Results VEGF, PDGF-B and ADAMTS-1 were significantly upregulated in the ischemic area in IPC group compared with the control group ( P < 0. 05 ).Density of mature arteries was also significantly increased in IPC plus MI group than that in MI group (P <0. 05). Conclusion IPC promoted the formation of mature new arteries which may be modulated by upregulating VEGF, PDGF-B, and ADAMTS-1 expressions.
