Value of in vivo monitoring of abdominal aortic atherosclerosis by high field magnetic resonance imaging in apoE -/- mice fed a high fat diet or infused with angiotensin Ⅱ
10.3760/cma.j.issn.0253-3758.2010.09.015
- VernacularTitle:小鼠腹主动脉斑块模型建立的两种方法及超高场强磁共振成像的应用价值
- Author:
Rui ZHAO
1
;
Yu-Yu YAO
;
Gang DENG
;
Sheng-Hong JU
;
Zhong-Juan WANG
;
Song WEN
;
Jun CHEN
;
Hui JIN
Author Information
1. 东南大学附属中大医院
- Keywords:
Atherosclerosis;
Magnetic resonance imaging;
Angiotensin Ⅱ;
Models,animal
- From:
Chinese Journal of Cardiology
2010;38(9):823-828
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the value of in vivo dynamic monitoring of abdominal aortic atherosclerosis (AS) by high field magnetic resonance (MR) imaging (MRI) in apoE - / - mice fed a high fat diet or infused with angiotensin. Methods High fat diet or angiotensin Ⅱ infusion was applied to apoE-/- mice for establishment of abdominal aortic atherosclerosis model. Abdominal aorta MRI was performed at 3 time points (baseline, 3 and 6 months) in 13 high fat diet fed apoE -/- mice aged 10 -12months and 3 wild-type control mice; 10 apoE -/- mice aged 6 months were infused with angiotensin Ⅱaortic artery MRI was performed at baseline and 14 d after infusion. Black blood sequences of FLASH T1 weighted images and Proton density weighted -T2 weighted dual echo images were obtained. At each observation time post MRI, mice (n =3, 5 and 5 for high fat diet group and n =5 and 5 for angiotensin Ⅱinfusion group) were sacrificed for pathological examination of the abdominal artery. Results ( 1 ) The abdominal aorta atherosclerosis was identified in both high fat diet and angiotensin Ⅱ treated apoE -/-mice but in WT controls. Lesion progression was documented in high fat diet fed apoE -/- mice characterized by significantly increased vessel wall( a marker of atherosclerotic burden, F = 29. 94, P < 0. 05 )and gradually increased plaque signal in PDW and T2W images. Results derived from MRI corresponded histopathology findings in high fat diet fed apoE -/- mice ( correlative coefficient = 0. 84, 0. 95, 0. 90,P < 0. 05, respectively). Both MRI and histology showed increased lipid composition and decreased fibrotic composition in these mice. (2) The vessel wall area increased significantly [(1.21 ±0.21) mm2 vs.(2. 65 ±0. 48 )mm2 ,P <0. 05] and the abdominal aortic dissection aneurysms was identified in apoE-/-mice infused with high angiotensin Ⅱ. The vessel wall area also increased [(0.85 ±0. 11) mm2 vs. ( 1.01 -0. 17) mm2, P <0. 05] in low angiotensin Ⅱ infused apoE -/- mice and the coefficient between MR and histopathology is 0. 934. Conclusion Abdominal aortic unstable plaque model could be established by both high fat diet and angiotensin Ⅱ infusion in apoE mice, angiotensin Ⅱ infusion can transiently accelerate the progression of AS and can induce abdominal aortic dissection. Serial MR black blood sequences could demonstrate the development and progression of atherosclerosis in mouse abdominal aorta with excellent agreement to histopathology finding in terms of atherosclerotic burden and plaque composition. Thus, MRI appears to be a useful tool for in vivo AS plaque dynamic monitoring in mice.
